Messi didn't play in Paris Saint-Germain's 2-2 draw at Red Bull Leipzig in the Champions League on Wednesday, but was still included by coach Lionel Scaloni for the games on November 12 and November 16.
Scaloni also included six young players who will feature in the national team for the first time.
Argentina has 25 points from 11 matches in South American qualifying and two more wins would put it on the brink of qualifying for next year's World Cup in Qatar.
Goalkeepers: Franco Armani (River Plate), Emiliano Martínez (Aston Villa), Juan Musso (Atalanta), Federico Gómes Gerth (Tigre, Argentina);
Defenders: Nahuel Molina (Udinese), Gonzalo Montiel, Marcos Acuña (Sevilla), Cristian Romero (Tottenham), Germán Pezzella (Betis), Nicolás Otamendi (Benfica), Lucas Martínez Quarta (Fiorentina), Nicolás Tagliafico, Lisandro Martínez (Ajax), Gastón Ávila (Rosario Central);
Midfielders: Guido Rodríguez (Betis), Leandro Paredes (Paris Saint-Germain), Enzo Fernández, Santiago Simón (River Plate), Rodrigo De Paul (Atlético Madrid), Exequiel Palacios (Bayer Leverkusen), Giovani Lo Celso (Tottenham), Nicolás Domínguez (Bologna), Cristian Medina (Boca Juniors), Matías Soulé (Juventus);
Forwards: Ángel Di María, Lionel Messi (Paris Saint-Germain), Ángel Correa (Atlético Madrid), Lautaro Martínez, Joaquín Correa (Inter Milan), Paulo Dybala (Juventus), Julián Álvarez (River Plate), Nicolás González (Fiorentina), Thiago Almada (Vélez Sarsfield), Exequiel Zeballos (Boca Juniors).